FASTQ to RAW files

Convert your FASTQ from any WGS DNA testing service to the most RAW common formats compatible with third-party DNA websites.

You will get the following results and files:

• RAW files compatible with all the known third-party DNA websites.
• BAM files ready to be uploaded to YFULL.com for deep YDNA analysis.
• MTDNA FASTA file ready to be uploaded to YFULL.com for deep MTDNA analysis.

Note:

• If your test is a FTDNA BIGY files, you will only receive the YDNA prediction and a VCF file containing the SNPs found in the BAM/CRAM file, as this analysis only provides the YDNA data.
• If your test is a Whole Exome Sequencing, due to its lower coverage compared to Whole Genome Sequencing (WGS), the resulting RAW files will contain fewer genotyped SNPs. Consequently, these files may not be accepted by third-party websites, and the ancestry estimates could be less accurate than a RAW file from a WGS analysis.

Required Files

To use our service, you need to provide the pair of FASTQ files.

You can share the required file by either:

1. Uploading the FASTQ file to Dropbox or Google Drive, and then sharing the link with us.
2. If you tested in Nebula Genomics, provide a direct link to the files from Nebula Genomics.

Important Notes

• MTDNA Information: The MTDNA data within Nebula Genomics’ CRAM files cannot be accurately converted into a RAW file, so the prediction from genealogy third-parties websites will not be accurate. However, the MTDNA FASTA and BAM files are correctly generated, and the MTDNA haplogroups in the MTDNA.txt file are reliably predicted.
• INDELs: Please note that INDELs (insertions and deletions) are not accurately converted into RAW files. Therefore, any Single Nucleotide Polymorphism (SNP) representing an INDEL will be incorrect.

DISCLAIMER
Please be advised that our Whole Genome Sequencing (WGS) to RAW data conversion services are specifically designed and intended for ancestry and genealogy research purposes only, not for health-related uses. We do not take responsibility for any health predictions made from any of the files provided through this service.

What files will I receive converting a FASTQ to RAW?

Here is the list of ready-to-use files you will receive when converting your WGS to RAW:

YDNA.TXT	Contains the predicted YDNA
MTDNA.txt	Contains the predicted MTDNA
RAW FILES
A RAW file compatible with the most know third-party DNA websites.	Recommended for known ancestry and genealogy websites
A 2 Millions SNPs RAW file that includes the most common SNPs from various DNA testing companies. This file is only compatible with certain third-party websites.	RAW file containing 2.5M SNPs. Recommended for GEDMatch, ...
MTDNA FILES
chrM.bam	BAM file of your MTDNA
chrM.vcf.gz	VCF file of your MTDNA
chrM.vcf.gz.tbi	VCF Index file of your VCF MTDNA file
mtdna.fasta	FASTA file of your MTDNA. Recommended to upload it to YFULL.com and James Lick MTDNA predictor
YDNA files. Only for Biological Men
chrY.annotated.vcf.gz	VCF file of your YDNA
chrY.annotated.vcf.gz.tbi	VCF Index file of your VCF YDNA file
chrY.bam	BAM file of your YDNA
chrYM.bam	BAM file of your YDNA and MTDNA. Recommended to upload it to YFULL.com to get matches and deeper prediction of your YDNA and MTDNA

What files will I receive if I convert a FTDNA BIGY?

If you convert a FTDNA BIGY file, you will receive a VCF file containing all the SNPs, which primarily includes YDNA data. This file will provide the specific variations in the Y chromosome, useful for tracing paternal lineage and ancestry.

How are the results delivered?

The results are delivered securely through your account, and all the data is removed once the customer confirms that they have downloaded it correctly.