Following these instructions you will create a 23andme RAW file that contains the variants of your VCF file. That is, the file will not contain the SNPs that are part of the Human Genome Reference, but just the SNP that are different from the Human Genome Reference.

Convert the Dante Lab’s VCF to a RAW “only variants”

You are going to create a 23andme V5 RAW file that will include only the variants (not the references values) that are in the Dante Lab’s VCF.

  1. Open DNA Kit Studio
  2. Choose the VCF to RAW option from the VCF Tools => VCF Converter tab
  3. Choose your gVCF/VCF file
  4. Choose a filename for the 23andme “only variants” raw file. It is suggested to follow the 23andme’s filename structure: genome_variants_NAMESURNAME_VX_DATE.txt
  5. Choose Match By Position
  6. Click on Convert and wait

Convert the Dante Lab’s VCF to a RAW containing VCF’s variants and a set of reference SNPs

You are going to create a 23andme V5 RAW file that will include the VCF’s variants from the VCF file and a set of reference data values.

Warning: Due to filtering options used by Dante Labs when created the VCF file, there could be many mismatches between your “real” SNP and the reference data leading to a poor matching or ethnicity estimation.

Use it own your risk.

  1. Open DNA Kit Studio
  2. Choose the VCF to RAW option from the VCF Tools => VCF Converter tab
  3. Choose your gVCF/VCF file
  4. Choose a filename for the 23andme “only variants” raw file. It is suggested to follow the 23andme’s filename structure: genome_variants_NAMESURNAME_VX_DATE.txt
  5. Choose Match By Position
  6. Click on Convert and wait
  7. Once the 23andme file is generated, go to the tab RAW Merger
  8. Choose first you generated 23andme file
  9. Then choose the file “genome_V5_MasterData.txt” in the folder “ReferenceData” of DNA Kit Studio
  10. Choose merge by RSID and uncheck “Add not common SNPs”